Supervisors:
Rodolphe Marie rcwm@nanotech.dtu.dk
Marie P Jensen
Project/group: Optofluidics
http://www.nanotech.dtu.dk/nil
Project description:
The optofluidic group has extensive experience with micro and nanofluidics. We are currently working on a microfluidic platform for single cell analysis. We have demonstrated that we can enrich cells using a microfluidic chip [1] and we also have developed a platform for capturing single cells.
In our project we have a very specific engineering issue of creating microfluidic devices with very high throughput meaning high flow rates or high number of cell trapping. This is non-trivial as some of the dimensions of our device cannot be enlarged as they match the dimensions of single cells. We thus need to change the way the devices are fabricated.
This project is about making a multilayer microfluidic chip using existing designs for separating and trapping single cells. You will be supervised for working with injection moulding, nano imprint lithography, experimental microfluidics and fluorescence microscopy.
References
1. Marie Pødenfandt, R. Marie, T. Olesen, M. Matteucci and A. Kristensen, “Injection molded pinched flow fractionation device for enrichment of somatic cells in cow milk”, Microelectronic Engineering, 2014 124, 53-57
Content
The project work will include:
- Fabricating a polymer device by injection moulding
- Making nanoimprint lithography
- Establishing a method for making a multilayer device
- Making sure the device works in a microfluidic experiment
Competencies
After this project you will:
- Achieve a simple fabrication process in the cleanroom
- Perform high quality fluorescence microscopy
