Contacts:
Dorota Kwasny dorota.kwasny@nantoech.dtu.dk
Karsten B. Andersen karsten.andersen@nanotech.dtu.dk
Background
Patients with impaired immunoprotection are exposed to high risk of infection. Infections that have a mild effect on a healthy individual can be fatal for many of these patients if the disease is not detected early enough. Fungal infections, especially by Candida albicans, are a major risk for the health of bone marrow transplant patients and their current diagnosis is time consuming and lacks accuracy.
Project description
In this project you will develop and fabricate a device for separation of yeast cells using a membrane based filtration technique. The device will be capable of processing blood sample to collect the yeast cells and to further separate blood into blood cells and plasma. The yeast collection efficiency will be analyzed optically as a function of the membrane pore size. The project will mostly include design, fast prototyping of the devices and experimental work.
In this project you will develop and fabricate a device for separation of yeast cells using a membrane based filtration technique. The device will be capable of processing blood sample to collect the yeast cells and to further separate blood into blood cells and plasma. The yeast collection efficiency will be analyzed optically as a function of the membrane pore size. The project will mostly include design, fast prototyping of the devices and experimental work.
Goal
The aim of the project is to develop a device for collection of Candida albicans using membrane based filtration of blood inside a microfluidic device. The initial design will be based on the preliminary work performed in the group with different pore-size membranes tested. Further development of the device is envisioned to create a functional device amenable for integration using a modular approach.
The aim of the project is to develop a device for collection of using membrane based filtration of blood inside a microfluidic device. The initial design will be based on the preliminary work performed in the group with different pore-size membranes tested. Further development of the device is envisioned to create a functional device amenable for integration using a modular approach.
You will be working with:
- Laser ablation prototyping
- Microfluidics
- Blood sample processing
- Yeast culturing